The Need

Mass spectrometry (MS) is a crucial analytical tool in proteomics, yet traditional MS experiments often face challenges such as complex ion generation processes and limited accessibility to samples during analysis. Existing solutions require multiple steps and modifications to instruments, which can be time-consuming and costly. The innovative micro-droplet interface technology addresses these issues by simplifying MS experiments through single-step ion generation and reaction processes external to the MS. This approach not only enhances the efficiency of MS experiments but also allows for easy collection and characterization of reaction products using various analytical methods, all without the need for instrument modifications. The technology is particularly impactful in applications requiring precise control of reaction times and in-situ modifications at atmospheric pressure, making it ideal for green synthesis and advanced proteomics analysis.

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The Technology

Researchers at Ohio State Innovation Foundation have developed a groundbreaking method for studying the reactivity of ionic species in a charged micro-droplet environment outside the mass spectrometer. This technology features a new reactive micro-droplet interface that processes electrospray droplets at atmospheric pressure, enabling green synthesis, enhanced reaction rates, and mixture analysis for proteomics purposes1. The core innovation lies in the contained-electrospray process, which permits effective control of residence time and in-situ modification of the droplet environment1. The apparatus includes three inlets for delivering liquid reagents, nebulizer gas, and headspace vapor of reactive gases into a single outlet that serves as a reaction cavity1. This design allows solution-phase reactants to be electrosprayed and held in a small reaction cavity as a liquid thin film, with reactants/products released by changes in solvent flow rate or nebulizer gas pressure1. The technology addresses current limitations by simplifying MS experiments and providing easy access to samples during analysis, making it adaptable for various applications in proteomics and green synthesis.

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Benefits

• Performance improvements: Accelerates reaction rates and enhances the efficiency of MS experiments.
• Cost-effectiveness: Reduces the need for instrument modifications and multiple-step processes.
• Versatility: Applicable in green synthesis and advanced proteomics analysis.
• Ease of use: Simplifies ion generation and reaction processes, allowing easy collection and characterization of samples.

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Patent

Issued US Utility Patent 9852896 . .