Butanol Production from Renewable Biomass
A genetically engineered bacteria pathway directed to selectively generate butanol over competing by-products.
Acetone-Butanol-Ethanol (ABE) Fermentation is one of two methods to produce butanol from biomass feedstocks. Various active enzymes or acids are exposed to a biomass feedstock and subsequently produce primarily acetone, butanol and ethanol. Previous attempts in the industry to use Clostridium strains to ferment butanol were plagued by high costs, relatively low-yields, sluggish fermentations, inhibition of the end product, and phage infections. Re-engineering Clostridium to increase yield of butanol over other undesired products would greatly improve biomass to product conversion efficiency.
Researchers at The Ohio State University, led by Dr. Shang-Tian Yang, have developed novel strains of Clostridium that produce significantly more n-butanol than their parent strains when exposed to biomass. These strains can be used for industrial biobutanol production to enable a more economical conversion of biomass feedstocks. The Clostridium strains are significantly more robust than previous conceptions and less sensitive to environmental factors. This durability enables the bacteria to digest higher quantities of biomass before dying, resulting in greater butanol generation compared to other mutant Clostridium strains.