Tethered Liposomal Nanoparticle (tLN) Microarray Chips Containing Molecular Probes for Simultaneous Cell Sorting and Bio DetectionA simple, and efficient method for identifying intra-cellular biomarkers for the targeted treatment of disease. The NeedTethered Lipoplex Nanoparticle (TLN) microarray biochips simultaneously capture and identify extracellular vesicles (EV) RNA targets from human plasma or bodily fluids with or without EV isolation. Molecular detection probes, such as molecular beacons (MBs), identify specific micro RNA (miRNA) or messenger RNA (mRNA). Currently, there is an unmet need for simple and inexpensive methods of capturing and identifying targeted cells alive, especially in body fluids, for disease treatment. The TechnologyResearchers at The Ohio State University, led by Dr. L. James Lee, have developed a method that utilizes surface receptors to capture target cells from cell mixtures and identify specific mRNAs and miRNAs as intra-cellular biomarkers without damaging the cells. The microarray biochip consists of tethered liposomal nanoparticles that contain molecular probes, such as MBs, inside the particles and antibodies on the particle surface. The microarray chips can also be used to capture and identify virus and cell secreted nanoparticles, such as microvesicles and exosomes. Commercial Applications
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Tech IDT2013-044 CollegeLicensing ManagerTaysavang, Panya InventorsCategories(None) |