The Need: In modern biotechnology and biomedical research, there is an escalating demand for efficient tools that can accurately detect and analyze specific RNA sequences. The ability to precisely target and identify RNA molecules, particularly U-rich sequences, is crucial for various applications such as gene expression analysis, RNA tracking, and disease diagnostics.

The Technology: Our groundbreaking technology introduces a novel peptide designed to N-terminally display either cyanine 3 (Cy3) or cyanine 5 (Cy5) dye, seamlessly coupled via an amide bond. This peptide sequence comprises an alternating pattern of serine and a unique lysine derivative, denoted as K*. K* is a lysine residue meticulously alkylated with two ethyl-melamine groups, strategically incorporated into the peptide using standard solid-phase peptide synthesis with Fmoc-K*.

Commercial Applications:

• Fluorescent labeling for RNA detection assays in gene expression studies.
• Bioanalytical tools for real-time monitoring of RNA interactions in cellular processes.
• Diagnostic probes for identifying specific RNA sequences associated with diseases.

Benefits/Advantages:

• Exceptional specificity: The tailored design ensures precise targeting of U-rich RNA sequences, minimizing off-target effects.
• High sensitivity: The fluorogenic nature of Cy3 or Cy5 dyes enables the detection of even low concentrations of RNA molecules.
• Versatile applications: From fundamental research to clinical diagnostics, the technology offers a wide range of potential uses in various fields of biotechnology and medicine.