CPSMV-based Gene Expression System-Enhancing the understanding of gene functions in cropsThe Need In the agricultural industry, soybean research has faced significant challenges in effectively interrogating gene functions due to the complex and time-consuming process of soybean transformation. The conventional methods of gene disruption and overexpression are hindered by technical difficulties, leading to slow progress and limited insights. An urgent need exists for a more efficient and adaptable technology that can enable rapid and transient gene silencing or overexpression to unlock the full potential of soybean research. The Technology Our solution, the CPSMV-derived FZ vector, presents a groundbreaking technology for soybean functional genomics. This vector is based on the cowpea severe mosaic virus (CPSMV), an innovative plus-strand RNA virus divided into two RNA segments, RNA1 and RNA2. The FZ vector incorporates a cloning site in the RNA2 cDNA, allowing the insertion of nonviral sequences. When coupled with an optimized RNA1 construct, the FZ vector efficiently infects both Nicotiana benthamiana and soybean plants. This technology enables the seamless transfer of modified viruses to high titers through N. benthamiana, ultimately targeting soybean and other crops, offering an unprecedented approach for gene silencing and overexpression studies. Commercial Applications
Benefits/Advantages
In conclusion, the CPSMV-derived FZ vector represents a game-changing technology for soybean functional genomics and plant research. By addressing the limitations of existing methods, this innovative tool promises to revolutionize the agricultural industry and drive advancements in crop improvement, biotechnology research, and sustainable food production. Embrace the future of soybean research with the FZ vector - unlocking the full potential of soybeans and beyond. |
Tech IDT2022-238 CollegeCollege of Food, Agricultural, and Environmental Sciences (CFAES) Licensing ManagerDahlman, Jason "Jay" InventorsCategories |