Tunable Fingerloop DNA antisense motifs for nucleic acid targeting and detection technologies

The Need: Improved Nucleic Acid Detection Probes

There is a growing need for advanced detection probes that can effectively identify target nucleic acid sequences while reducing binding to mismatched targets. Existing technologies often suffer from false positive results due to temperature fluctuations or imperfect base pairing. There is a demand for a more reliable and specific nucleic acid detection method that can be used for various applications, including point-of-care diagnostics and nucleic acid amplification.

The Technology: Fingerloop Antisense Sequence Motif

Fingerloop is a novel structural antisense sequence motif inspired by the regulatory sRNA DsrA found in E. coli. The technology involves a single-stranded inverted repeat sequence forming a stem-loop, with the antisense sequence residing in one strand. This motif has two alternative forms, the ascending stem, and the descending stem. The structural stability of the helix can be fine-tuned by adjusting the length of the helix, the size of the loop, and the introduction of mismatches or non-Watson-Crick base pairs.

Commercial Applications:

  • Nucleic Acid Detection: Fingerloop technology enables highly specific and accurate nucleic acid detection, making it suitable for various diagnostic applications.
  • Point-of-Care Diagnostics: The technology's portability and temperature independence allow it to be used for point-of-care testing, eliminating the need for centralized testing facilities.
  • Nucleic Acid Amplification: Fingerloop structures can be incorporated into amplification primers, enhancing the specificity and fidelity of nucleic acid amplification techniques like PCR.
  • Modulation of Protein Expression: The technology's ability to modulate protein expression levels can be applied in gene expression studies and other research areas.
  • mRNA Expression Measurement: Fingerloop structures can help measure mRNA expression levels from multiple target mRNAs simultaneously.


  • Enhanced Specificity: Fingerloop technology significantly reduces off-target interactions and false positive results, ensuring accurate and reliable nucleic acid detection.
  • Temperature Independence: The technology's stability is maintained despite temperature changes, making it highly suitable for various environmental conditions.
  • Versatility: Fingerloop structures can be adapted for diverse applications, including diagnostics, gene expression studies, and more.
  • No Enzyme Dependency: Unlike some detection methods, fingerloop diagnostics rely almost exclusively on nucleic acid derivatives, simplifying the testing process.
  • Improved Amplification Fidelity: By incorporating fingerloop structures into amplification primers, the fidelity and specificity of nucleic acid amplification techniques are greatly enhanced.

In summary, Fingerloop Antisense Technology represents a breakthrough in nucleic acid detection, offering a highly specific, stable, and versatile solution for various commercial applications. Its advantages lie in its temperature independence, reduced false positives, and adaptability for point-of-care diagnostics, nucleic acid amplification, and gene expression modulation. Embrace the future of precise and reliable nucleic acid detection with Fingerloop.

Loading icon