Method and device for determining virus titer by surface enhanced Raman scattering
The ability to rapidly identify and determine virus titer is of utmost importance in numerous biomedical applications, ranging from gene editing to pharmaceutical and vaccine development. In the context of lentiviruses, which are widely used for cell reprogramming in immunotherapies, knowing the effective titer of the transformed virus is crucial to determining the appropriate dosage and expectations for therapy. Current methods for characterizing viruses and determining viral titer, such as ELISA, PCR, and cell culture, although reliable, suffer from disadvantages like time-consuming procedures and extensive sample preparation, leading to delays in obtaining results. There is a critical commercial need for a technology that can provide quicker and more straightforward determination of virus titer to accelerate pharmaceutical and vaccine development processes.
The technology being presented is a method for determining virus titer in a sample using Raman spectroscopy. Surface-Enhanced Raman Spectroscopy (SERS) is employed, which utilizes plasmonic metallic nanostructures to enhance the Raman signal of the virus in the sample. This enhancement enables the acquisition of a molecular fingerprint based on the virus's vibrational modes, facilitating the identification and quantification of the virus titer.
The technology has broad commercial applications in various fields, including:
The technology offers several key benefits and advantages:
In conclusion, the technology's ability to rapidly and directly determine virus titer using SERS holds immense potential for revolutionizing various biomedical fields and expediting critical processes in pharmaceutical and vaccine development. Its efficiency, versatility, and reduced sample preparation make it a powerful tool for researchers and practitioners seeking to enhance their virus characterization and quantification capabilities.